A direct-staining method to evaluate the mucoadhesion of polymers from aqueous dispersion

Kockisch, S., Rees, G.D., Young, S.A., Tsibouklis, J. and Smart, J.D. (2001) A direct-staining method to evaluate the mucoadhesion of polymers from aqueous dispersion Journal of Controlled Release, 77 (1-2). pp. 1-6.

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Abstract

A novel technique to evaluate polymer adhesion to human buccal cells following exposure to aqueous polymer dispersion, both in vitro and in vivo, is described. Adhering polymer has been visualised by staining with 0.1% (w/v) of either Alcian blue (60 min) or Eosin (10 min) solution, uncomplexed dye being removed by 0.25 M sucrose washings. The extent of polymer adhesion was quantified by measuring the relative staining intensity of control and polymer-treated cells by image analysis. In vitro, Carbopol 974P, polycarbophil (Noveon AA-1) and chitosan (CL 113) were found to adhere to human buccal cells from 0.10% (w/w) aqueous dispersions of these polymers. Following in vivo administration as a mouthwash, these polymers persisted upon the human buccal mucosa for at least 1 h.

Item Type: Journal article
Uncontrolled Keywords: Bioadhesion; Oral cavity; Poly(acrylic acid); Chitosan
Subjects: B000 Health Professions > B200 Pharmacology Toxicology and Pharmacy
DOI (a stable link to the resource): 10.1016/S0168-3659(01)00444-8
Faculties: Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences > Microbiology
Depositing User: editor spbs
Date Deposited: 01 Dec 2006
Last Modified: 11 Jun 2013 11:13
URI: http://eprints.brighton.ac.uk/id/eprint/962

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