High-porosity activated carbon as a possible matrix for native DNA and dextran-sulfate immobilization

Snezkova, E.A., Muller, D., Bardakhivskaya, K.L., Mikhalovsky, S.V. and Nikolaev, V.G. (2004) High-porosity activated carbon as a possible matrix for native DNA and dextran-sulfate immobilization Artificial Cells, Blood Substitutes and Biotechnology, 32 (4). pp. 529-537. ISSN 1532-4184

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Abstract

In this study, specific and nonspecific activity of calf thymus DNA and Dextran-Sulfate (DS)-containing synthetic carbonic adsorbents (0.7–7 mg of each ligands per 1 cm 3 of activated carbonic beads, 0.3–0.6 mm diameter, bulk density γ = 0.1–0.2 g/cm 3) have been compared in stir-bath and micro-column in vitro tests. DS coating as well as DNA coating does not demonstrate deep influence on the unspecific adsorptive activity of carbonic matrix toward creatinine, vitamin B 12, and unconjugated bilirubin. No essential difference has been found in the specific activity of DNA and DS containing adsorbents toward anti-ds- and anti-ss-DNA-antibodies, as well as antibodies against DNA-protein complexes (anti-DNP-antibodies): in both cases the percentage of decrease of appropriate antibody concentration varied between 35 and 51% for single-pass microcolumn experiments with moderate enhancement of extraction efficacy (up to 60–75%) due to additional recirculation (2 h) or preliminary plasma dilution in 2–5 times. In the micro-column experiments with the proinflammatory cytokines DNA or DS-coating did not diminish TNF-α, IL-1β, and IL-6 adsorption from 3% BSA solution, but even improves to some extent its removal compared with uncoated matrix.

Item Type: Journal article
Uncontrolled Keywords: Carbonic adsorbents; Fractality; Dextran-sulfate; DNA; Cytokines
Subjects: C000 Biological and Biomedical Sciences
DOI (a stable link to the resource): 10.1081/BIO-200039612
Faculties: Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences
Depositing User: editor spbs
Date Deposited: 01 Dec 2006
Last Modified: 25 Apr 2014 15:16
URI: http://eprints.brighton.ac.uk/id/eprint/922

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