High-porosity activated carbon as a possible matrix for native DNA and dextran-sulfate immobilization

Abstract

In this study, specific and nonspecific activity of calf thymus DNA and Dextran-Sulfate (DS)-containing synthetic carbonic adsorbents (0.7–7 mg of each ligands per 1 cm 3 of activated carbonic beads, 0.3–0.6 mm diameter, bulk density γ = 0.1–0.2 g/cm 3) have been compared in stir-bath and micro-column in vitro tests. DS coating as well as DNA coating does not demonstrate deep influence on the unspecific adsorptive activity of carbonic matrix toward creatinine, vitamin B 12, and unconjugated bilirubin. No essential difference has been found in the specific activity of DNA and DS containing adsorbents toward anti-ds- and anti-ss-DNA-antibodies, as well as antibodies against DNA-protein complexes (anti-DNP-antibodies): in both cases the percentage of decrease of appropriate antibody concentration varied between 35 and 51% for single-pass microcolumn experiments with moderate enhancement of extraction efficacy (up to 60–75%) due to additional recirculation (2 h) or preliminary plasma dilution in 2–5 times. In the micro-column experiments with the proinflammatory cytokines DNA or DS-coating did not diminish TNF-α, IL-1β, and IL-6 adsorption from 3% BSA solution, but even improves to some extent its removal compared with uncoated matrix.