Characteristics of interfacial water affected by proteins adsorbed on activated carbon
Alekseeva, T.A., Lebovka, N.I., Gun'ko, V.M., Strashko, V.V. and Mikhalovsky, S.V. (2004) Characteristics of interfacial water affected by proteins adsorbed on activated carbon Journal of colloid and interface science, 278 (2). pp. 333-341. ISSN 0021-9797
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Official URL: http://dx.doi.org/10.1016/j.jcis.2004.06.030
The influence of proteins (bovine serum albumin, BSA, and mouse γ-globulin, IgG) physically adsorbed or covalently attached via coupling with N-cyclohexyl-N′-(2-morpholinoethyl) carbodiimide methyl-p-toluenesulfonate, CMC, to the surface of activated carbon SCN (spherical carbon with nitrogen) on the mobility of interfacial water molecules was studied by means of 1H NMR spectroscopy with freezing-out of bulk water at 180<T<273 K. Relaxation processes in the interfacial non-freezing water were investigated measuring transverse time t2 of proton relaxation dependence on the presence of proteins and CMC. The distribution function of activation free energy of relaxation (with a maximum at 20–22 kJ/mol) was calculated for the protein–water–carbon systems using a regularization procedure and the relationships between t2 and the amounts of the interfacial water unfrozen at T<250 K assuming the Arrhenius-type dependence for t2−1 on temperature. The state of unfrozen water in pores of SCN shows that the low temperature relaxation processes occur in narrow pores with half-width X<1.5 nm.
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