Alekseeva, T.A., Lebovka, N.I., Gun'ko, V.M., Strashko, V.V. and Mikhalovsky, Sergey (2004) Characteristics of interfacial water affected by proteins adsorbed on activated carbon Journal of Colloid and Interface Science, 278 (2). pp. 333-341.Full text not available from this repository.
The influence of proteins (bovine serum albumin, BSA, and mouse γ-globulin, IgG) physically adsorbed or covalently attached via coupling with N-cyclohexyl-N′-(2-morpholinoethyl) carbodiimide methyl-p-toluenesulfonate, CMC, to the surface of activated carbon SCN (spherical carbon with nitrogen) on the mobility of interfacial water molecules was studied by means of 1H NMR spectroscopy with freezing-out of bulk water at 180<T<273 K. Relaxation processes in the interfacial non-freezing water were investigated measuring transverse time t2 of proton relaxation dependence on the presence of proteins and CMC. The distribution function of activation free energy of relaxation (with a maximum at 20–22 kJ/mol) was calculated for the protein–water–carbon systems using a regularization procedure and the relationships between t2 and the amounts of the interfacial water unfrozen at T<250 K assuming the Arrhenius-type dependence for t2−1 on temperature. The state of unfrozen water in pores of SCN shows that the low temperature relaxation processes occur in narrow pores with half-width X<1.5 nm.
|Item Type:||Journal article|
|Uncontrolled Keywords:||Activated carbon; Microporosity; Surface properties; 1H NMR spectroscopy; Nonfreezable interfacial water; Protein immobilization; Protein chemisorption|
|Subjects:||F000 Physical Sciences|
|DOI (a stable link to the resource):||10.1016/j.jcis.2004.06.030|
|Faculties:||Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences|
|Depositing User:||editor spbs|
|Date Deposited:||01 Dec 2006|
|Last Modified:||30 Mar 2015 10:55|
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