Boronate-containing polymer brushes: characterization, interaction with saccharides and mammalian cancer cells

Ivanov, A.E., Eccles, J., Ahmad Panahi, H., Kumar, A., Kuzimenkova, M.V., Nillson, L., Bergenståhl, B., Long, N., Phillips, G.J., MIKHALOVSKY, SERGEY, Yu Galaev, I. and Mattiasson, B. (2009) Boronate-containing polymer brushes: characterization, interaction with saccharides and mammalian cancer cells Journal of Biomedical Materials Research Part A, 88 (1). pp. 213-25. ISSN 1549-3296

Full text not available from this repository.

Official URL: http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.3...

Abstract

Boronate-containing polymer brushes were synthesized by free radical copolymerization of N,N-dimethylacrylamide (DMAA) and N-acryloyl-m-phenylboronic acid (NAAPBA) (9:1) on the surface of 3-mercaptopropyl-silylated glass plates and capillaries. The brushes were characterized with time-of-flight secondary ion mass-spectrometry (ToF SIMS), atomic force microscopy and contact angle measurements. Fructose caused a well-expressed drop spreading on the surface of copolymer-grafted glass, due to the strong interaction with the boronate groups. Sedimentation of murine hybridoma cells M2139 or human myeloid leukemia cells KG1 onto the DMAA-NAAPBA copolymer-grafted glass plates from 10 mM phosphate buffer solution (pH 8.0) resulted in the cell adhesion. The adhered M2139 and KG1 cells could be quantitatively detached from the grafted plates with 0.1M fructose, which competed with cell surface carbohydrates for binding to the boronates. Evaluation of the binding strength between M2139 cells and the copolymer brush was performed by exposure of the adhered cells to a shear stress. Detachment of a fraction of 18% of the adhered M2139 cells was obtained at a shear force of 1400-2800 pN/cell generated by the running phosphate buffer (pH 8.0), whereas the remaining adhered cells (70%) could be detached with 0.1M fructose dissolved in the same buffer. Possible applications of the boronate-containing polymer brushes to affinity cell separation can be based upon the facile recovery of the attached cells. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res 2008

Item Type:Journal article
Subjects:C000 Biological and Biomedical Sciences
C000 Biological and Biomedical Sciences > C700 Molecular Biology, Biophysics and Biochemistry
DOI (a stable link to the resource):10.1002/jbm.a.31880
Faculties:Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences > Biomedical Materials
Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences
ID Code:8020
Deposited By:Converis
Deposited On:22 Dec 2010 15:50
Last Modified:05 Apr 2013 13:58

Repository Staff Only: item control page