Suppression of intestinal polyposis in Apcmin/+ mice by targeting the nitric oxide or poly(ADP-ribose) pathways

Mabley, J.G., Pacher, P., Bai, P., Wallace, R., Goonesekera, S., Virag, L., Southan, G.J. and Szabo, C. (2004) Suppression of intestinal polyposis in Apcmin/+ mice by targeting the nitric oxide or poly(ADP-ribose) pathways Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 548 (1-2). pp. 107-116. ISSN 0027-5107

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Abstract

Min mice have a germ-line nonsense mutation at codon 850 of the adenomatous polyposis coli (Apc) gene. These mice spontaneously develop multiple polyps in the small and large intestine at the age of 10–12 weeks. The aim of this study was to assess the role of reactive nitrogen species and poly(ADP-ribose) synthetase in tumorogenesis. Oxidative stress was found to be increased in the mucosa of the small intestine of Apcmin/+ mice with a concomitant increase in intestinal polyposis over control mice. Pharmacological inhibition of inducible nitric oxide synthase (NOS) with guanidinoethyldisulfide (GED) or stimulation of the breakdown of the nitrogen reactive species peroxynitrite using a potent decomposition catalyst, FP 15, reduced both the intestinal tumor load and the oxidative stress associated with intestinal polyposis in Apcmin/+ mice. Surprisingly, pharmacological inhibition of poly(ADP-ribose) synthetase by the phenanthridinone derivative PJ 34 also reduced the intestinal polyposis and oxidative stress in these mice, possibly through the inhibition of induction of nitric oxide synthase. These results suggest that reactive nitrogen species particularly peroxynitrite play a pivotal role in development of intestinal polyposis and that strategies to reduce both the oxidative stress and the formation of these radical species may be potential chemopreventive approaches for colorectal cancers.

Item Type: Journal article
Uncontrolled Keywords: Apcmin/+ mice; Oxidative stress; Peroxynitrite; Nitric oxide; Poly(ADP-ribose) synthetase
Subjects: C000 Biological and Biomedical Sciences
DOI (a stable link to the resource): 10.1016/j.mrfmmm.2004.01.006
Faculties: Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences
Depositing User: editor spbs
Date Deposited: 01 Dec 2006
Last Modified: 17 Apr 2014 14:33
URI: http://eprints.brighton.ac.uk/id/eprint/795

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