Continuous amperometric detection of co-released serotonin and melatonin from the mucosa in the ileum

Patel, B.A. (2008) Continuous amperometric detection of co-released serotonin and melatonin from the mucosa in the ileum Analyst, 133 (4). pp. 516-524. ISSN 0003-2654

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Official URL: http://pubs.rsc.org/en/content/articlelanding/2008...

Abstract

Serotonin (5-HT) and melatonin (MEL) are well known neurotransmitters and paracrine signalling molecules. Both compounds are present in enterochromaffin (EC) cells in the mucosa of the gastrointestinal tract and are thought to play a role in controlling gut motility. To date there are no real-time analytical methods for the detection of these two molecules and it is not clear if MEL is actually released from the EC cells. In this paper, I used boron-doped diamond (BDD) microelectrodes to record 5-HT and MEL overflow from EC cells in the mucosa of rabbit ileum. The BDD microelectrode was extremely stable and sensitive for measurements of both compounds when assessed using differential pulse voltammetry (DPV) and flow injection analysis (FIA) using amperometric detection. MEL release was detected in the mucosa, where it is most likely from the EC cells. Mechanical stimulation of individual villi increased 5-HT but not MEL overflow. Application of the serotonin transporter (SERT) inhibitor, fluoxetine, elevated the 5-HT but not the MEL signal. Differences in the amounts of the two gastrointestinal compounds released and the mechanism of which they are released will provide insights to the physiology of the EC cell and disease states.

Item Type:Journal article
Subjects:C000 Biological and Biomedical Sciences
DOI (a stable link to the resource):10.1039/B717034C
Faculties:Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences
ID Code:7692
Deposited By:editor spbs
Deposited On:17 Sep 2010 14:50
Last Modified:17 May 2012 02:18

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