Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates
van der Valk, A.M., Howbrook, D.N., O'Shaughnessy, M.C., Sarker, D.K., Baker, S.C., Louwrier, A. and Lloyd, A.W. (2003) Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates Biotechnology Letters, 25 (16). pp. 1325-1328. ISSN 1573-6776
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Official URL: http://link.springer.com/article/10.1023%2FA%3A102...
The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.
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