Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates

van der Valk, A.M., Howbrook, D.N., O'Shaughnessy, M.C., Sarker, D.K., Baker, S.C., Louwrier, A. and Lloyd, A.W. (2003) Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates Biotechnology Letters, 25 (16). pp. 1325-1328. ISSN 1573-6776

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Abstract

The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.

Item Type: Journal article
Uncontrolled Keywords: alkaline phosphatase; biotin; fluorescein; polypropylene; streptavidin
Subjects: C000 Biological and Biomedical Sciences
DOI (a stable link to the resource): 10.1023/A:1024951322955
Faculties: Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences > Biomedical Materials
Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences
Depositing User: editor spbs
Date Deposited: 29 Nov 2006
Last Modified: 06 Nov 2013 16:29
URI: http://eprints.brighton.ac.uk/id/eprint/751

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