van der Valk, A.M., Howbrook, D.N., O'Shaughnessy, M.C., Sarker, D.K., Baker, S.C., Louwrier, A. and Lloyd, A.W. (2003) Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates Biotechnology Letters, 25 (16). pp. 1325-1328. ISSN 1573-6776Full text not available from this repository.
The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.
|Item Type:||Journal article|
|Uncontrolled Keywords:||alkaline phosphatase; biotin; fluorescein; polypropylene; streptavidin|
|Subjects:||C000 Biological and Biomedical Sciences|
|DOI (a stable link to the resource):||10.1023/A:1024951322955|
|Faculties:||Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences > Biomedical Materials
Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences
|Depositing User:||editor spbs|
|Date Deposited:||29 Nov 2006|
|Last Modified:||06 Nov 2013 16:29|
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