Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates

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van der Valk, A.M., Howbrook, D.N., O'Shaughnessy, M.C., Sarker, D.K., Baker, S.C., Louwrier, A. and Lloyd, A.W. (2003) Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates Biotechnology letters, 25 (16). pp. 1325-1328. ISSN 1573-6776

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Official URL: http://dx.doi.org/10.1023/A:1024951322955

Abstract

The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.

Item Type:	Journal article
Uncontrolled Keywords:	alkaline phosphatase - biotin - fluorescein - polypropylene - streptavidin
Subjects:	C000 Biological and Biomedical Sciences
DOI (a stable link to the resource):	10.1023/A:1024951322955
Faculties:	Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences > Biomedical Materials Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences
ID Code:	751
Deposited By:	editor spbs
Deposited On:	29 Nov 2006
Last Modified:	18 Jun 2010 12:29

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