Hardy, S.P., Ritchie, C., Allen, Marcus, Ashley, R.H. and Granum, P.E. (2001) Clostridium perfringens type A enterotoxin forms mepacrine-sensitive pores in pure phospholipid bilayers in the absence of putative receptor proteins BBA - Biomembranes, 1515 (1). pp. 38-43. ISSN 0005-2736Full text not available from this repository.
Clostridium perfringens enterotoxin (CPE) is an important cause of food poisoning with no significant homology to other enterotoxins and its mechanism of action remains uncertain. Although CPE has recently been shown to complex with tight junction proteins, we have previously demonstrated that CPE increases ionic permeability in single Caco-2 cells using the whole-cell patch-clamp technique, thereby excluding any paracellular permeability. In this paper we demonstrate that CPE forms pores in synthetic phospholipid membranes in the absence of receptor proteins. The properties of the pores are consistent with CPE-induced permeability changes in Caco-2 cells suggesting that CPE has innate pore-forming ability.
|Item Type:||Journal article|
|Uncontrolled Keywords:||Food poisoning; Pore-forming toxin; Planar lipid bilayer|
|Subjects:||C000 Biological and Biomedical Sciences > C700 Molecular Biology, Biophysics and Biochemistry|
|DOI (a stable link to the resource):||10.1016/S0005-2736(01)00391-1|
|Faculties:||Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences|
|Depositing User:||editor spbs|
|Date Deposited:||08 Nov 2007|
|Last Modified:||05 Mar 2015 14:42|
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