A rapid, non-destructive method for the determination of Staphylococcus epidermidis adhesion to surfaces using quartz crystal resonant sensor technology
Pavey, K.D., Barnes, L.M., Hanlon, G.W., Olliff, C.J., Ali, Z. and Paul, F. (2001) A rapid, non-destructive method for the determination of Staphylococcus epidermidis adhesion to surfaces using quartz crystal resonant sensor technology Letters in Applied Microbiology, 33 (5). pp. 344-348. ISSN 1365-2673
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Official URL: http://onlinelibrary.wiley.com/doi/10.1046/j.1472-...
Aims: To investigate the use of quartz crystal resonant sensor (QCRS) technology to determine the adhesion of Staphylococcus epidermidis to fibronectin-coated surfaces. Methods and Results: QCRS sensors (14 MHz) with 4 mm gold electrodes were coated with fibronectin and exposed for 15 min to suspensions of Staph. epidermidis ranging in concentration from 1 × 102 to 1 × 106 cfu ml-1. Changes in resonant frequency were recorded and showed a linear relationship with the logarithm of cell concentration over the range tested. Conclusions: QCRS technology was shown to be a rapid, sensitive and non-destructive method for measuring the adhesion of bacteria to surfaces. Significance and Impact of the Study: This report demonstrates that QCRS technology has the potential to be used for a range of applications requiring measurement of bacteria on surfaces. In particular, it may be used for the real-time monitoring of bacterial biofilm formation.
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