A rapid, non-destructive method for the determination of Staphylococcus epidermidis adhesion to surfaces using quartz crystal resonant sensor technology

Pavey, K.D., Barnes, Lara-Marie, Hanlon, Geoff, Olliff, C.J., Ali, Z. and Paul, F. (2001) A rapid, non-destructive method for the determination of Staphylococcus epidermidis adhesion to surfaces using quartz crystal resonant sensor technology Letters in Applied Microbiology, 33 (5). pp. 344-348. ISSN 1365-2673

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Abstract

Aims: To investigate the use of quartz crystal resonant sensor (QCRS) technology to determine the adhesion of Staphylococcus epidermidis to fibronectin-coated surfaces. Methods and Results: QCRS sensors (14 MHz) with 4 mm gold electrodes were coated with fibronectin and exposed for 15 min to suspensions of Staph. epidermidis ranging in concentration from 1 × 102 to 1 × 106 cfu ml-1. Changes in resonant frequency were recorded and showed a linear relationship with the logarithm of cell concentration over the range tested. Conclusions: QCRS technology was shown to be a rapid, sensitive and non-destructive method for measuring the adhesion of bacteria to surfaces. Significance and Impact of the Study: This report demonstrates that QCRS technology has the potential to be used for a range of applications requiring measurement of bacteria on surfaces. In particular, it may be used for the real-time monitoring of bacterial biofilm formation.

Item Type: Journal article
Subjects: C000 Biological and Biomedical Sciences > C500 Microbiology
B000 Health Professions > B800 Medical Technology > B830 Biomaterials and Prosthetics
A000 Medicine > A300 Clinical Medicine
DOI (a stable link to the resource): 10.1046/j.1472-765X.2001.01009.x
Faculties: Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences > Microbiology
Depositing User: editor spbs
Date Deposited: 08 Nov 2007
Last Modified: 19 Mar 2015 14:59
URI: http://eprints.brighton.ac.uk/id/eprint/592

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