The synergistic effect of EDTA/antimicrobial combinations on Pseudomonas aeruginosa

Lambert, R.J.W., Hanlon, G.W. and Denyer, S.P. (2004) The synergistic effect of EDTA/antimicrobial combinations on Pseudomonas aeruginosa Journal of Applied Microbiology, 96 (2). pp. 244-253.

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Abstract

Aims: To demonstrate that the nonlinear concentration-dependent inhibition of Pseudomonas aeruginosa to EDTA can be used to successfully model and predict the potentiation of antimicrobials by EDTA. Methods and Results: A model used successfully to describe the concentration-dependent inhibition of bacterial growth caused by many antimicrobials was unable to describe the inhibition of P. aeruginosa by EDTA. Examination of the inhibition profiles for EDTA against P. aeruginosa revealed a biphasic inhibitory pattern suggesting different mechanisms of action at different concentrations. A modelled, two-stage inhibitory process was shown to fit the observations. This model was then used to examine the effect of combining EDTA with other antimicrobials. The apparent synergy of mixtures of EDTA with quaternary ammonium surfactants (QAC) and specific antibiotics was successfully modelled. Minimum inhibitory concentrations (MIC) of the QAC and that of oxacillin and cefamandole were reduced by a factor of 3–10, whereas ampicillin was reduced by a factor of 70 from an MIC of 1524 to 21 mg l-1 in the presence of 500 mg l-1 of EDTA. Conclusions: A nonlinear concentration-dependent inhibition of P. aeruginosa by EDTA gives rise to apparent observation of synergy with other antimicrobials. Significance and Impact of the Study:This is a further example where the current methodology for the examination of antimicrobial synergy (the summed fractional inhibitory concentrations) leads to false conclusions.

Item Type: Journal article
Uncontrolled Keywords: antibiotic combination; potentiation; preservation; synergy
Subjects: C000 Biological and Biomedical Sciences > C500 Microbiology
DOI (a stable link to the resource): 10.1046/j.1365-2672.2004.02135.x
Faculties: Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences > Microbiology
Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences
Depositing User: editor spbs
Date Deposited: 29 Nov 2006
Last Modified: 06 Jun 2013 09:05
URI: http://eprints.brighton.ac.uk/id/eprint/588

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