A rat monoclonal antibody that catalyses the hydrolysis of a nitrophenyl-β-lactam
Ostler, E.L., Dean, C.J., Barber, N., Valeri, M., James, S., Resmini, M., Boucher, G., Romanov, N., Brocklehurst, K. and Gallacher, G. (2002) A rat monoclonal antibody that catalyses the hydrolysis of a nitrophenyl-β-lactam Biochemical and biophysical research communications, 299 (2). pp. 273-276. ISSN 0006-291X
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Official URL: http://dx.doi.org/10.1016/S0006-291X(02)02604-9
We report the first example of a monoclonal antibody-catalysed hydrolysis of a β-lactam where the antibodies were generated by a simple transition-state analogue. A rat monoclonal antibody (1/91c/4d/26) generated by using an acyclic 4-nitrophenylphosphate immunogen catalysed the hydrolysis of corresponding 4-nitrophenyl carbonates but, more importantly, also catalysed the hydrolysis of N-(4-nitrophenyl)-azetidinone at pH 8 with kcat=8.7×10−6 s−1 and KM=35 μM. This is the first example of a rat monoclonal catalytic antibody.
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