Novel quartz crystal microbalance based biosensor for detection of oral epithelial cell-microparticulate interaction in real-time

LETHEM, MIKE, ELSOM, JACQUELINE, Rees, G.D. and Hunter, A.C. (2008) Novel quartz crystal microbalance based biosensor for detection of oral epithelial cell-microparticulate interaction in real-time Biosensors & Bioelectronics, 23 (8). pp. 1259-1265. ISSN 0956-5663

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Abstract

Recent applications of quartz crystal resonant sensor technology to monitor cell adhesion and specific ligand interaction processes has triggered the development of a new category of quartz crystal microbalance (QCM) based biosensors. In this study human oral epithelial cells (H376) were cultured on quartz sensors and their response to microspheres investigated in situ using the QCM technique. The results demonstrated that this novel biosensor was able to follow cell–microsphere interactions in real-time and under conditions of flow as would occur in the oral cavity. Unique frequency profiles generated in response to the microspheres were postulated to be due to phases of mass addition and altered cellular rigidity. Supporting microscopic evidence demonstrated that the unique frequency responses obtained to these interactions were in part due to binding between the cell surface and the microspheres. Furthermore, a cellular uptake process, in response to microsphere loading was identified and this, by influencing the rigidity of the cellular cytoskeleton, was also detectable through the frequency responses obtained.

Item Type: Journal article
Uncontrolled Keywords: Quartz crystal microbalance; Biosensor; Oral epithelial cells; Cell culture; Real-time
Subjects: C000 Biological and Biomedical Sciences > C700 Molecular Biology, Biophysics and Biochemistry
F000 Physical Sciences > F100 Chemistry
DOI (a stable link to the resource): 10.1016/j.bios.2007.11.020
Faculties: Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences > Chemical Biology
Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences
Depositing User: Converis
Date Deposited: 12 Mar 2008
Last Modified: 05 Apr 2013 11:23
URI: http://eprints.brighton.ac.uk/id/eprint/4726

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