Characterization of a putative nitric oxide synthase in the neuromuscular system of the parasitic nematode, Ascaris suum

Bascal, Z.A., Cunningham, J.M., Holden-Dye, L., O'Shea, M. and Walker, R.J. (2001) Characterization of a putative nitric oxide synthase in the neuromuscular system of the parasitic nematode, Ascaris suum Parasitology, 122 (2). pp. 219-232. ISSN 1469-8161

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Abstract

In this paper we report on the biochemical presence of nitric oxide synthase (NOS)-like activity in Ascaris suum tissue and examine the pharmacological effect of NO donors on A. suum muscle strip preparation. NOS activity was determined by monitoring the formation of [3H]citrulline from [3H]L-arginine and NO formation via the oxyhaemoglobin assay. Neuromuscular tissue from A. suum which stained positively for NADPH diaphorase, contained NOS activity. Neither NOS activity nor NADPH diaphorase staining was detected in intestinal tissue. The absence of Ca2+, NADPH and other co-factors normally required for mammalian neuronal NOS activity only partially reduced the formation of both citrulline and NO by A. suum neuromuscular homogenate. The results of the biochemical assays indicate the presence of an enzyme capable of producing NO and citrulline, but with a different profile from that of rat neuronal NOS. We also present preliminary evidence for the action of NO (NO donors) in the neuromuscular system of A. suum.

Item Type: Journal article
Uncontrolled Keywords: Ascaris suum; nematode; invertebrate; nitric oxide synthase.
Subjects: C000 Biological and Biomedical Sciences
DOI (a stable link to the resource): 10.1017/S003118200100720X
Faculties: Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences
Depositing User: editor spbs
Date Deposited: 08 Nov 2007
Last Modified: 18 Jun 2010 11:28
URI: http://eprints.brighton.ac.uk/id/eprint/469

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