Linoleic acid and antioxidants protect against DNA damage and apoptosis induced by palmitic acid

Beeharry, N., Lowe, J.E., Hernandez, A.R., Chambers, J.A., Fucassi, F., Cragg, Peter, Green, M.H.L. and Green, I.C. (2003) Linoleic acid and antioxidants protect against DNA damage and apoptosis induced by palmitic acid Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 530 (1-2). pp. 27-33. ISSN 0027-5107

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Polyunsaturated fats are the main target for lipid peroxidation and subsequent formation of mutagenic metabolites, but diets high in saturated fats are more strongly associated with adverse health effects. We show that the common saturated fatty acid, palmitic acid, is a potent inducer of DNA damage in an insulin-secreting cell line, and in primary human fibroblasts. Damage is not associated with upregulation of inducible nitric oxide synthase, but is prevented by two different antioxidants, α-lipoic acid and 3,3′-methoxysalenMn(III) (EUK134), which also partly prevent palmitic acid-induced apoptosis and growth inhibition. Since mutagenic metabolites can be formed from peroxidation of polyunsaturated fatty acids, co-administration of palmitic and a polyunsaturated fatty acid might be particularly harmful. Palmitic acid-induced DNA damage is instead prevented by linoleic acid, which is acting here as a protective agent against oxidative stress, rather than as a source of mutagenic metabolites. These results illustrate the complexity of the relationship of dietary fat intake to genotoxicity.

Item Type: Journal article
Uncontrolled Keywords: Salen antioxidants; Comet assay; Insulin-producing cells; Human fibroblasts; Free fatty acids
Subjects: C000 Biological and Biomedical Sciences > C700 Molecular Biology, Biophysics and Biochemistry
DOI (a stable link to the resource): 10.1016/S0027-5107(03)00134-9
Faculties: Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences
Depositing User: editor spbs
Date Deposited: 08 Nov 2007
Last Modified: 18 Mar 2015 16:17

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