Inhibition of Nonproteolytic Clostridium botulinum wit Lactic Acid Bacteria and Their Bacteriocins at Refrigeration Temperatures

Abstract

Nonproteolytic Clostridium Botulinum (strains 17B, Beluga, and 202F) was found to be inhibited by Lactobacillus, Lactococcus, Streptococcus and Pediococcus species in tests by the spot-on-the-lawn similtaneous-antagonism method at 10, 15 amd 25 degree celsius. C. botullinum 17B was the most resitant strain. Inhibition zone size increased with decreasing incubation temperatrure. Six strains of Lactobacillus acidphillus and seven strains of bifidobacteria failed to produce an inhibiton zone on buffered reinforced clostridium prussain blue agar seeded with spores of any of the selected C. botulinum strains. C. botulinum 17B was sensitive to 50 to 100 IU of nisin per ml and 10 to 20 AU of pediocin A per ml.