Direct capture of plasmid DNA from non-clarified bacterial lysate using polycation-grafted monoliths

Hanora, A, Savina, I.N., Plieva, F.M, Izumrudov, V.A, Mattiasson, B and Galaev, I.Y (2006) Direct capture of plasmid DNA from non-clarified bacterial lysate using polycation-grafted monoliths Journal of Biotechnology, 123 (3). pp. 343-355. ISSN 0168-1656

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Abstract

Monolith columns from macroporous polyacrylamide gel were grafted with polycations, poly(N,N-dimethylaminoethyl methacrylate) (polyDMAEMA), (2-(methacryloyloxy)ethyl)-trimethyl ammonium chloride (polyMETA) and partially quaternized polyDMAEMA prepared via treating polyDMAEMA-grafted columns with propylbromide. The polymer grafting degrees varied between 34 and 110%. The polycation-grafted monolithic columns are able to capture plasmid DNA directly from alkaline lysate of Escherichia coli cells. Due to the large pore size in macroporous monoliths the particulate material present in non-clarified feeds did not block the columns. The captured plasmid DNA was eluted with 1 M NaCl as particulate-free preparation with significantly reduced content of protein and RNA as compared to the applied lysate.

Item Type: Journal article
Subjects: C000 Biological and Biomedical Sciences > C700 Molecular Biology, Biophysics and Biochemistry
DOI (a stable link to the resource): 10.1016/j.jbiotec.2005.11.017
Faculties: Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences > Biomedical Materials
Depositing User: editor spbs
Date Deposited: 11 Oct 2007
Last Modified: 16 May 2012 14:09
URI: http://eprints.brighton.ac.uk/id/eprint/2718

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