Hanlon, G.W., Denyer, S.P., Hodges, N.A., Brant, J.A., Lansley, A.B. and Al-Rustamani, W.A. (2004) Biofilm formation and changes in bacterial cell surface hydrophobicity during growth in a CAPD model system Journal of Pharmacy and Pharmacology, 56 (7). pp. 847-854. ISSN 0022-3573Full text not available from this repository.
Peritonitis is a frequent complication of continuous ambulatory peritoneal dialysis (CAPD), with patients suffering recurrent attacks. The microorganisms most frequently implicated in the infection are the skin microflora, in particular, the coagulase-negative staphylococci such as Staphylococcus epidermidis. These microorganisms gain access to the peritoneal cavity via the in-dwelling silicone rubber catheter in the abdominal wall and often persist as biofilms on the surface of the catheter. The surface characteristics of S. epidermidis were monitored during growth in a CAPD in-vitro model together with their ability to adhere to silicone rubber substrata. Fresh dialysis fluid exerted an injurious effect on the cells leading to a decrease in cell numbers but during the simulated dialysis period the cells adapted to the applied stresses. Over a 96-h period in the model both a clinical isolate and a skin isolate of S. epidermidis adopted a more hydrophobic phenotype. The data presented here show that the bacteria grown in this in-vivo reflective CAPD model continually adapt to their environment and become more tolerant to the stresses imposed. The adapted cells were seen to colonise silicone rubber substrata.
|Item Type:||Journal article|
|Subjects:||C000 Biological and Biomedical Sciences|
|DOI (a stable link to the resource):||10.1211/0022357023817|
|Faculties:||Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences|
|Depositing User:||editor spbs|
|Date Deposited:||08 Nov 2007|
|Last Modified:||02 May 2014 13:56|
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