Altered composition and DNA binding activity of the AP-1 transcription factor during the ageing of human fibroblasts

Sheerin, A., Thompson, K.S.J. and Goyns, M.H. (2001) Altered composition and DNA binding activity of the AP-1 transcription factor during the ageing of human fibroblasts Mechanisms of Ageing and Development, 122 (15). pp. 1813-1824. ISSN 0047-6374

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Abstract

We have investigated the expression of AP-1 transcription factor proteins during the in-vitro ageing of human fibroblasts. The numbers of these cells that are in the cell cycle gradually decreases up to 45 cumulative population doublings (cPD), thereafter the decline is steeper, until almost all cells enter a post-mitotic state by 60 cPD. We observed that a 34 kd junB species began to replace the 44 kd junB species after 41 cPD. This was followed, after 44 cPD, by a loss of fra1 and both junD species. After 49 cPD there was a gradual decline in the levels of fos and jun proteins, but disproportionately, so that the fos/jun protein ratio also declined. Although fos and jun proteins were still clearly present at 60 cPD, utilisation of the AP-1 DNA consensus sequence could not be demonstrated after 54 cPD. These data indicate that significant changes occur in the composition of the AP-1 transcription factor during ageing, but also that alterations in its DNA binding activity may involve other factors.

Item Type: Journal article
Subjects: C000 Biological and Biomedical Sciences
DOI (a stable link to the resource): 10.1016/S0047-6374(01)00319-0
Faculties: Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences
Depositing User: editor spbs
Date Deposited: 08 Nov 2007
Last Modified: 07 Nov 2014 15:40
URI: http://eprints.brighton.ac.uk/id/eprint/2614

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