Calcium-binding phospholipids as a coating material for implant osteointegration

Santin, Matteo, Rhys-Williams, W., O'Reilly, J., Davies, M.C., Shakesheff, K., Love, W.G., Lloyd, Andrew and Denyer, Stephen (2006) Calcium-binding phospholipids as a coating material for implant osteointegration Journal of the Royal Society Interface, 3 (7). pp. 277-281. ISSN 1742-5689

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Among the many biomolecules involved in the bone mineralization processes, anionic phospholipids play an important role because of their ability to bind calcium. In particular, phosphatidylserine is a natural component of the plasmalemma and of the matrix vesicles generated from the osteoblast membrane to create nucleation centres for calcium phosphate crystal precipitation. In the present work, we demonstrate that calcium-binding phospholipids can be used as biomimetic coating materials for improving the osteointegration of metal implants. Relatively thick phosphatidylserine-based coatings were deposited on titanium coupons by dip-coating. Upon dehydration in a simulated body fluid phospholipids were quickly crosslinked by calcium and re-arranged into a three-dimensional matrix able to induce rapid formation of a calcium phosphate mineral phase. The rate of mineralization was shown to be dependent on the adopted coating formulation. In the attempt to closely mimic the cell membrane composition, heterogeneous formulations based on the mixing of anionic phospholipids (either phosphatidylserine or phosphatidylinositol) with phosphatidylcholine and cholesterol were synthesized. However, surface plasmon resonance studies as well as scanning electron microscopy and elemental analysis demonstrated that the homogeneous phosphatidylserine coating was a more effective calcification environment than the heterogeneous formulations.

Item Type: Journal article
Uncontrolled Keywords: biomaterials; phospholipids; coatings; mineralization; osteointegration
Subjects: F000 Physical Sciences > F200 Materials Science
C000 Biological and Biomedical Sciences
C000 Biological and Biomedical Sciences > C700 Molecular Biology, Biophysics and Biochemistry
DOI (a stable link to the resource): 10.1098/rsif.2005.0088
Faculties: Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences
Depositing User: editor spbs
Date Deposited: 08 Nov 2007
Last Modified: 08 Dec 2016 10:45

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