Activation of poly(ADP-ribose) polymerase in circulating leukocytes during myocardial infarction

Murthy, K.G.K, Xiao, C.Y, Mabley, J.G, Chen, M and Szabo, C (2004) Activation of poly(ADP-ribose) polymerase in circulating leukocytes during myocardial infarction Shock, 21 (3). pp. 230-234. ISSN 1073-2322

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Official URL: http://dx.doi.org/10.1097/01.shk.0000110621.42625....

Abstract

Myocardial ischemia-reperfusion can lead to increased oxidative stress both locally and in circulating leukocytes. Oxidant-mediated DNA single strand breaks are known to activate the nuclear enzyme poly(ADP-ribose) polymerase (PARP) in various forms of shock, inflammation, and ischemia-reperfusion injury. The aim of the current study was to investigate whether a local insult such as myocardial ischemia-reperfusion is sufficient to lead to activation of PARP in circulating leukocytes. In anesthetized rats myocardial ischemia-reperfusion was induced by transient ligation of the left anterior descending coronary artery. There was a marked increase in poly(ADP-ribosyl)ation of proteins in homogenates of leukocytes isolated from rats at the end of the reperfusion period. Poly(ADP-ribosyl)ation was inhibited by administration of the pharmacologic PARP inhibitor INO-1001 (30 mg/kg) to the rats. We conclude that local insults, such as myocardial reperfusion injury, are sufficient to activate PARP in circulating leukocytes. PARP activation in circulating cells may mediate certain systemic effects of local ischemia-reperfusion injury such as inflammatory mediator production and remote organ injury.

Item Type:Journal article
Subjects:C000 Biological and Biomedical Sciences
DOI (a stable link to the resource):10.1097/01.shk.0000110621.42625.10
Faculties:Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences
ID Code:2588
Deposited By:editor spbs
Deposited On:08 Nov 2007
Last Modified:18 Jun 2010 12:33

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