Cooper, Ian, Taylor, Huw and Hanlon, Geoff (2007) Virulence traits associated with VTEC Escherichia coli O157 recovered from freshwater biofilms. Journal of Applied Microbiology, 102 (5). pp. 1293-1299.Full text not available from this repository.
Aim: To investigate whether epilithic biofilms in freshwater streams in a mixed UK agricultural river catchment harbour Escherichia coli O157, and if so, whether they demonstrate an association with those excreted by grazing farm animals. Methods and Results: Flint shingle, native to the study site, was used as a surface for biofilm development within cages of metal lath set into a stream bed at four locations on a chalkland farm. Shingle was collected from all sites once a month, as were pooled faecal samples from five farm animal populations. Subpopulations of E. coli, including E. coli O157 that demonstrated significant phenotypic and genotypic similarity with animal faecal isolates (t-test, P = 0·05) were isolated. Of 1002 E. coli isolates from biofilms and animal faeces, 48 were confirmed as the O157 strain by latex agglutination. The presence of five virulence traits associated with incidence of human disease was tested using PCR. Stx2 was the most frequently isolated single gene (30 isolates), while stx1 was the least frequently recovered (four isolates). Conclusion: Escherichia coli O157, expressing up to four virulence factors associated with human disease, reside within freshwater biofilms in this agricultural environment. Significance and Impact of the Study: Aquatic biofilms may potentially act as a reservoir for these pathogens, and the implications of the findings for the protection of drinking water resources should be further investigated.
|Item Type:||Journal article|
|Uncontrolled Keywords:||biofilm, E. coli O157, faeces, PCR, phenotyping, water|
|Subjects:||C000 Biological and Biomedical Sciences > C500 Microbiology|
|DOI (a stable link to the resource):||10.1111/j.1365-2672.2006.03178.x|
|Faculties:||Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences > Microbiology|
|Depositing User:||Ian Cooper|
|Date Deposited:||08 Nov 2007|
|Last Modified:||16 Mar 2015 14:19|
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