Catalytic superoxide scavenging by metal complexes of the calcium chelator EGTA and contrast agent EHPG

Fisher, A.E.O., Hague, T.A., Clarke, C.L. and Naughton, D.P. (2004) Catalytic superoxide scavenging by metal complexes of the calcium chelator EGTA and contrast agent EHPG Biochemical and biophysical research communications, 323 (1). pp. 163-7. ISSN 0006-291X

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Official URL: http://dx.doi.org/10.1016/j.bbrc.2004.08.066

Abstract

Metal ion chelators widely used in experimental protocols and clinical diagnosis are generally assumed to be inert. We previously reported that the ubiquitous chelator EDTA has high levels of superoxide suppressing activity. Here, we report that the common chelators calcium chelator EGTA and contrast agent EHPG have significant activities in suppressing superoxide levels depending on the nature of metal ion chelated. The most active species is Mn(II)-EGTA which exhibited an IC50 value of 0.19 microM for superoxide destruction. In addition, IC50 values for Mn(II)-EHPG and 2Cu(II)-EGTA were 0.69 and 0.60 microM, respectively. In conclusion, Mn(II) and Cu(II) complexes of the common chelators EGTA and EHPG exhibit considerable superoxide scavenging activities. Caution should be employed in their use in biological systems where superoxide has a key role and they may be useful for the development of catalytic anti-oxidants.

Item Type:Journal article
Uncontrolled Keywords:Chelator; Superoxide dismutase; Metal ions; Ethylenebis(hydroxyphenylglycine); Ethylenebis(oxyethylenenitrilo)tetraacetic acid
Subjects:B000 Health Professions > B200 Pharmacology Toxicology and Pharmacy > B250 Pharmacodynamics and Pharmacokinetics
DOI (a stable link to the resource):10.1016/j.bbrc.2004.08.066
Faculties:Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences
ID Code:20
Deposited By:editor spbs
Deposited On:15 Oct 2007
Last Modified:18 Jun 2010 12:27

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