Leukocyte Hsp72 mRNA transcription does not differ between males and females during heat acclimation

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Mee, Jessica, Gibson, Oliver, Tuttle, J.A., Taylor, L., Watt, Peter, Doust, Jonathan and Maxwell, Neil (2016) Leukocyte Hsp72 mRNA transcription does not differ between males and females during heat acclimation Temperature, 3 (4). pp. 549-556. ISSN 2332-8959

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Abstract

Purpose: Thermotolerance is an acquired state of increased cytoprotection achieved following single or repeated exposures to heat stress, in part characterised by changes in the intracellular 72kda heat shock protein (HSP72; HSPA1A). Females have demonstrated reduced exercise induced HSP72 in comparison to males. This study examined sex differences in heat shock protein 72 messenger ribonucleic acid (Hsp72 mRNA) transcription during heat acclimation (HA) to identify whether sex differences were a result of differential gene transcription.  Methods: Ten participants (5M, 5F) performed ten, 90 min controlled hyperthermia [rectal temperature (Tre) ≥ 38.5°C] HA sessions over 12 d. Leukocyte Hsp72 mRNA was measured pre and post D1, D5, and D10, via Reverse transcription polymerase chain reaction (RT-QPCR).  Results: HA was evidenced by a reduction in resting Tre (-0.4 ± 0.5°C) and resting heart rate [(HR); -13 ± 7 beats.min-1] following HA (p ≤ 0.05). During HA no difference (p > 0.05) was observed in ΔTre between males (D1 = 1.5 ± 0.2°C; D5 = 1.6 ± 0.4°C; D10 = 1.8 ± 0.3°C) and females (D1 = 1.5 ± 0.5°C; D5 = 1.4 ± 0.2°C; D10 = 1.8 ± 0.3°C). This was also true of mean Tre demonstrating equality of thermal stimuli for mRNA transcription and HA. There were no differences (p > 0.05) in Hsp72 mRNA expression between HA sessions or between males (D1 = +1.8 ± 1.5 fold; D5 = +2.0 ± 1.0 fold; D10 = +1.1 ± 0.4 fold) and females (D1 = +2.6 ± 1.8 fold; D5 = +1.8 ± 1.4 fold; D10 = +0.9 ± 1.9 fold).  Conclusions: This experiment demonstrates that there is no difference in Hsp72 mRNA increases during HA between sexes when controlled hyperthermia HA is utilised. Gender specific differences in exercise-induced HSP72 reported elsewhere likely result from post-transcriptional events.

Item Type: Journal article
Additional Information: This is an Accepted Manuscript of an article published by Taylor & Francis in Temperature on 27/07/2016, available online: http://www.tandfonline.com/10.1080/23328940.2016.1214336.
Uncontrolled Keywords: Controlled hyperthermia; males; females; thermotolerance; heat shock protein
Subjects: C000 Biological and Biomedical Sciences > C600 Sport and Exercise Science
L000 Social Sciences > L300 Sociology > L311 Sport and Leisure
B000 Health Professions > B100 Anatomy Physiology and Pathology > B120 Physiology
B000 Health Professions > B100 Anatomy Physiology and Pathology
B000 Health Professions > B100 Anatomy Physiology and Pathology > B190 Anatomy, Physiology and Pathology not elsewhere classified
DOI (a stable link to the resource): 10.1080/23328940.2016.1214336
Depositing User: Converis
Date Deposited: 05 Aug 2016 03:02
Last Modified: 27 Jul 2017 00:38
URI: http://eprints.brighton.ac.uk/id/eprint/15882

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