Simple and rapid determination of serotonin and catecholamines in biological tissue using high-performance liquid chromatography with electrochemical detection

Patel, B.A., Arundell, M., Parker, K.H., Yeoman, M.S. and O'Hare, D. (2005) Simple and rapid determination of serotonin and catecholamines in biological tissue using high-performance liquid chromatography with electrochemical detection Journal of chromatography B, 818 (2). pp. 269-276. ISSN 1570-0232

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1016/j.jchromb.2005.01.008

Abstract

Using the CNS of Lymnaea stagnalis a method is described for the rapid analysis of neurotransmitters and their metabolites using high performance liquid chromatography coupled with electrochemical detection. Tissue samples were homogenised in ice-cold 0.1 M perchloric acid and centrifuged. Using a C18 microbore column the mobile phase was maintained at a flow rate of 100 μl/min and consisted of sodium citrate buffer (pH 3.2)–acetonitrile (82.5:17.5, v/v) with 2 mM decane-sulfonic acid sodium salt. The potential was set at +750 mV versus Ag|AgCl reference electrode at a sensitivity of 50 nA full scale deflection. The detection limit for serotonin was 11.86 ng ml−1 for a 5 μl injection. Preparation of tissue samples in mobile phase reduced the response to dopamine and serotonin compared with perchloric acid. In addition it was found that the storage of tissue samples at −20 °C caused losses of dopamine and serotonin. As a result of optimising the sample preparation and mobile phase the total time of analysis was substantially reduced resulting in a sample preparation and assay time of 15–20 min.

Item Type:Journal article
Uncontrolled Keywords:Microbore HPLC; Serotonin; Catecholamines; Lymnaea stagnalis
Subjects:C000 Biological and Biomedical Sciences
DOI (a stable link to the resource):10.1016/j.jchromb.2005.01.008
Faculties:Faculty of Science and Engineering > School of Pharmacy and Biomolecular Sciences
ID Code:1052
Deposited By:editor spbs
Deposited On:01 Dec 2006
Last Modified:17 Sep 2010 15:17

Repository Staff Only: item control page